Analysis of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus trans-activators IE-1 and IE-2 using monoclonal antibodies
نویسندگان
چکیده
منابع مشابه
Location and nucleotide sequence of the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus polyhedrin gene.
A restriction endonuclease map was determined for the Orgyia pseudotsugata single nucleocapsid nuclear polyhedrosis virus (SNPV) genome. The order of the fragments generated by the enzymes BglII, BamHI and XbaI was analysed using double digestion of the total genome and digestion of isolated restriction fragments. The location of the polyhedrin gene was then determined using a cloned polyhedrin...
متن کاملProduction of polyhedrin monoclonal antibodies for distinguishing two Orgyia pseudotsugata baculoviruses.
Monoclonal antibodies were produced to polyhedrins from Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) and single-capsid nuclear polyhedrosis virus (OpSNPV). Although the polyhedrins are closely related, antibodies were selected which allowed differentiation between the two viruses. In an indirect enzyme-linked immunosorbent assay, purified OpMNPV and OpSNPV polyhedrins co...
متن کامل2. IE & IEs
PROTO-INDO-EUROPEAN is the traditional name given to the ancestor language of the Indo-European family that is spread from Iceland to Chinese Turkestan and from Scandinavia to the Near East. A PROTO-LANGUAGE (Gk. prõtos ‘first’) refers to the earliest form of a language family presupposed by all of its descendants. There will forever be major gaps in the reconstruction of proto-languages, but a...
متن کاملInhibitor of apoptosis protein from Orgyia pseudotsugata nuclear polyhedrosis virus provides a costimulatory signal required for optimal proliferation of developing thymocytes.
The inhibitors of apoptosis proteins (IAPs) constitute a family of endogenous inhibitors that control apoptosis in the cell by inhibiting caspase processing and activity. IAPs are also implicated in cell division, cell cycle regulation, and cancer. To address the role of IAPs in thymus development and homeostasis, we generated transgenic mice expressing IAP generated from the baculovirus Orgyia...
متن کاملAbilist Ie
In this, paper we present a probabilistic approach to analysis and prediction of protein structure. We argue that this approach provides a flexible and convenient mechanism to perform general scientific data analysis in molecular biology. We apply our approach to an important problem in molecular biology-predicting the secondary structure of proteins-and obtain experimental results comparable t...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1993
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-74-9-1819